Abstract
Mycoplasma pneumoniae initiates infection in the human host by attachment to respiratory epithelium. The organism attaches by a specialized terminal structure. Monoclonal antibodies to an organism surface protein (P1) inhibited attachment to respiratory epithelium and were localized to the tip structure by a ferritin antibody label. The P1 protein was degraded by trypsin treatment to smaller polypeptides that possessed the same antigenic determinants as the larger P1 protein when reacted with the specific monoclonal antibody, and evidence has been provided for the existence of multiple antigenic determinants on the attachment protein.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adhesins, Bacterial*
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Adhesiveness
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Animals
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Antibodies, Monoclonal
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Bacterial Proteins / analysis*
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Bacterial Proteins / immunology*
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Bacterial Proteins / isolation & purification
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Epithelium / microbiology
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Epitopes / analysis*
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Humans
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Membrane Proteins / analysis*
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Membrane Proteins / immunology
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Mice
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Mice, Inbred BALB C
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Mycoplasma pneumoniae / analysis*
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Mycoplasma pneumoniae / immunology
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Mycoplasma pneumoniae / ultrastructure
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Trachea / microbiology
Substances
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Adhesins, Bacterial
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Antibodies, Monoclonal
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Bacterial Proteins
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Epitopes
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Membrane Proteins
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adhesin, Mycoplasma pneumoniae