An immunohistochemical study of adenomatoid tumors utilizing keratin and factor VIII antibodies. Evidence for a mesothelial origin

Lab Invest. 1982 Sep;47(3):276-80.

Abstract

Fifteen adenomatoid tumors were examined immunohistochemically using antibody probes to keratin and factor VIII-related antigen. Cells lining adenoid structures labeled using keratin antibody in all 15 tumors but failed to label with factor VIII-related antigen antibodies in any case. In one case tissue was retrieved from a paraffin block and studied by immunoelectron microscopy using the two antibody probes. Immunoprecipitate was demonstrated in adenoid cells adjacent to desmosomes using keratin antibody but was absent using factor VIII-related antigen antibodies. Of the two prevailing theories of origin for adenomatoid tumors, based on ultrastructural and histochemical data to date, these results are additional evidence for a mesothelial origin and fail to support an endothelial origin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoma / pathology*
  • Adult
  • Antibodies*
  • Enzyme-Linked Immunosorbent Assay
  • Factor VIII / immunology*
  • Female
  • Horseradish Peroxidase
  • Humans
  • Immunoenzyme Techniques
  • Keratins / immunology*
  • Male
  • Middle Aged

Substances

  • Antibodies
  • Keratins
  • Factor VIII
  • Horseradish Peroxidase