RNA Modification Regulatory Genes in DNA Damage

Methods Mol Biol. 2019:1870:249-262. doi: 10.1007/978-1-4939-8808-2_19.

Abstract

Expression of genetic information is a multistep process which needs to be tightly regulated. One of the regulatory mechanisms is posttranscriptional modification of RNA, which can alter the stability, expression, or protein composition. Therefore, misregulation of this important cellular process can lead to pathological consequences, such as cancer development. It has been shown that alteration in the expression of certain RNA-modifying genes can promote tumorigenesis. Here, we present a mRNA expression analysis-based approach to comprehensively determine the expression of RNA readers/writers/erasers using DNA damage as an example, and then to validate the effect of altered RNA reader/writer/erasers in regulating the DNA damage response.

Keywords: DNA damage; Gene expression analysis; RNA modification.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / pharmacology
  • DNA Damage* / drug effects
  • DNA Damage* / radiation effects
  • Gamma Rays
  • Gene Expression Regulation* / drug effects
  • Gene Expression Regulation* / radiation effects
  • Humans
  • RNA / genetics*
  • RNA Processing, Post-Transcriptional / drug effects
  • RNA Processing, Post-Transcriptional / radiation effects
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Antineoplastic Agents
  • RNA, Messenger
  • RNA