Optical Transfection

Cold Spring Harb Protoc. 2018 Dec 3;2018(12). doi: 10.1101/pdb.top096222.

Abstract

Strategies for the delivery of genes into eukaryotic cells fall into three categories: transfection by biochemical methods, transfection by physical methods, and virus-mediated transduction. "Optical transfection"-a physical transfection method-exploits the ability of light to create small transient pores in the plasma membrane of mammalian cells.

MeSH terms

  • Animals
  • Cell Membrane / radiation effects*
  • Eukaryotic Cells / radiation effects*
  • Humans
  • Mammals
  • Optical Devices*
  • Optical Phenomena*
  • Transfection / methods*