Strontium phosphate transfection of human cells in primary culture: stable expression of the simian virus 40 large-T-antigen gene in primary human bronchial epithelial cells

Mol Cell Biol. 1987 May;7(5):2031-4. doi: 10.1128/mcb.7.5.2031-2034.1987.

Abstract

Strontium ion formed DNA-phosphate precipitates analogous to those formed by calcium but lacking the lethal and differentiation-inducing effects of calcium on many epithelial cell types in primary culture. Human primary bronchial epithelial cells were transiently and stably transfected by using strontium phosphate; the frequency of stable transformation with a plasmid carrying the simian virus 40 large-T-antigen gene was greater than 10(-4).

MeSH terms

  • Antigens, Viral, Tumor / genetics*
  • Bronchi / cytology
  • Bronchi / physiology*
  • Cells, Cultured
  • Chemical Precipitation
  • Epithelial Cells
  • Gene Expression Regulation
  • Genes, Viral
  • Humans
  • Phosphates*
  • Simian virus 40
  • Strontium*
  • Transfection*

Substances

  • Antigens, Viral, Tumor
  • Phosphates
  • strontium phosphate
  • Strontium