Abstract
In the process of developing [18F]FBEM coupled target peptide, we have instituted a robust automated synthesis of [18F]FBEM, a sulfhydryl (-SH) site specific agent for radiolabeling of peptides and proteins. The radiosynthesis generated 1.67-3.89 GBq (45.1-105.1 mCi, 7.5-18.8% non-decay corrected yield) of [18F]FBEM from 22.2 GBq (600 mCi) of starting [18F]fluoride with molar activity of 31.8 ± 5.3 GBq/µmol (0.86 ± 0.14 mCi/nmol) (n = 3) at the end of synthesis. Radiochemical purity was greater than 98%, and total synthesis time was ~90 min.
Keywords:
Automation; PET; Radiotracer synthesis; Thiol(-SH)-specific; [(18)F]FBEM.
Copyright © 2018 Elsevier Ltd. All rights reserved.
MeSH terms
-
Animals
-
Chromatography, High Pressure Liquid
-
Fluorine Radioisotopes / chemistry*
-
Glucagon-Like Peptide 1 / analogs & derivatives*
-
Glucagon-Like Peptide 1 / chemical synthesis
-
Glucagon-Like Peptide 1 / chemistry
-
Glucagon-Like Peptide 1 / standards
-
Maleimides / chemical synthesis*
-
Maleimides / chemistry*
-
Maleimides / standards
-
Peptides / chemistry
-
Proteins / chemistry
-
Quality Control
-
Radiochemistry / instrumentation
-
Radiochemistry / methods
-
Radiopharmaceuticals / chemical synthesis*
-
Radiopharmaceuticals / chemistry*
-
Radiopharmaceuticals / standards
-
Sulfhydryl Reagents / chemical synthesis
-
Sulfhydryl Reagents / chemistry
Substances
-
Fluorine Radioisotopes
-
Maleimides
-
N-2-(4-fluorobenzamido)ethylmaleimide-EM 3106B
-
Peptides
-
Proteins
-
Radiopharmaceuticals
-
Sulfhydryl Reagents
-
Glucagon-Like Peptide 1
-
Fluorine-18