Generation of Ultra-Small PLGA Nanoparticles by Sequential Centrifugation

Xingwang Wu; Jiangbing Zhou; Toral R Patel

doi:10.1007/978-1-4939-8661-3_2

Generation of Ultra-Small PLGA Nanoparticles by Sequential Centrifugation

Methods Mol Biol. 2018:1831:17-24. doi: 10.1007/978-1-4939-8661-3_2.

Authors

Xingwang Wu^{1

2}, Jiangbing Zhou¹, Toral R Patel³

Affiliations

¹ Department of Neurosurgery, Yale University, New Haven, CT, USA.
² Department of Radiology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
³ Department of Neurological Surgery, UT Southwestern Medical Center, Dallas, TX, USA. toral.patel@utsouthwestern.edu.

PMID: 30051421
DOI: 10.1007/978-1-4939-8661-3_2

Abstract

Direct, local delivery of polymer nanoparticles to the brain is a promising strategy to bypass the blood-brain barrier (BBB) and safely deliver a large therapeutic payload. However, even with the aid of convection-enhanced delivery (CED) techniques, this approach has been limited by the inability to fabricate appropriately sized polymer nanoparticles. Here, we outline a versatile and efficient method for producing polymer nanoparticles that are <100 nm in diameter and can be delivered to the brain via CED.

Keywords: Blood-brain barrier; Brain; Convection-enhanced delivery; Nanoparticle; PLGA; Polymer.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Centrifugation / methods*
Nanoparticles / chemistry*
Nanoparticles / ultrastructure
Particle Size*
Polylactic Acid-Polyglycolic Acid Copolymer / chemistry*

Substances

Polylactic Acid-Polyglycolic Acid Copolymer