Scarless genome editing: progress towards understanding genotype-phenotype relationships

Curr Genet. 2018 Dec;64(6):1229-1238. doi: 10.1007/s00294-018-0850-8. Epub 2018 Jun 5.

Abstract

The ability to predict phenotype from genotype has been an elusive goal for the biological sciences for several decades. Progress decoding genotype-phenotype relationships has been hampered by the challenge of introducing precise genetic changes to specific genomic locations. Here we provide a comparative review of the major techniques that have been historically used to make genetic changes in cells as well as the development of the CRISPR technology which enabled the ability to make marker-free disruptions in endogenous genomic locations. We also discuss how the achievement of truly scarless genome editing has required further adjustments of the original CRISPR method. We conclude by examining recently developed genome editing methods which are not reliant on the induction of a DNA double strand break and discuss the future of both genome engineering and the study of genotype-phenotype relationships.

Keywords: CRISPR; Genome editing; Genotype-Phenotype relationships.

Publication types

  • Review

MeSH terms

  • Animals
  • CRISPR-Cas Systems
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • DNA Breaks, Double-Stranded
  • Gene Editing*
  • Genetic Association Studies
  • Genetic Engineering*
  • Genotype
  • Humans
  • Phenotype