TAT-conjugated chitosan cationic micelle for nuclear-targeted drug and gene co-delivery

Colloids Surf B Biointerfaces. 2018 Feb 1:162:326-334. doi: 10.1016/j.colsurfb.2017.11.066. Epub 2017 Dec 5.

Abstract

We developed a high-efficiency nucleus-targeted co-delivery vector that delivers genes and drugs directly into the nucleus of cancer cells. The system is based on grafted poly-(N-3-carbobenzyloxy-lysine) (CPCL) with transactivator of transcription (TAT)- chitosan on the surface. It is designed to perform highly efficient nucleus- targeted gene and drug co-delivery. Confocal laser scanning microscopy (CLSM) revealed that more TAT-CPCL entered the nucleus than does CPCL alone. The TAT-modified vector serves as a gene and drug co-delivery mechanism to achieve high gene transfection efficiency, high apoptosis and low viability in HeLa cells. TAT-CPCL may become a vector for cancer gene treatment and a template for designing better co-deliver systems.

Keywords: Cationic micelle; Chitosan; Co-delivery; Nucleus-targeted; TAT.

MeSH terms

  • Antibiotics, Antineoplastic / pharmacology
  • Apoptosis / drug effects
  • Cell Nucleus / drug effects*
  • Cell Nucleus / metabolism
  • Chitosan / chemistry*
  • Doxorubicin / pharmacology
  • Drug Carriers*
  • Gene Products, tat / genetics
  • Gene Products, tat / metabolism*
  • Gene Transfer Techniques*
  • Genetic Vectors / chemistry*
  • Genetic Vectors / metabolism
  • HeLa Cells
  • Humans
  • Micelles
  • Particle Size
  • Polylysine / chemistry
  • Surface Properties

Substances

  • Antibiotics, Antineoplastic
  • Drug Carriers
  • Gene Products, tat
  • Micelles
  • Polylysine
  • Doxorubicin
  • Chitosan