Visualization and quantification of injury to the ciliated epithelium using quantitative flow imaging and speckle variance optical coherence tomography

Ute A Gamm; Brendan K Huang; Emily K Mis; Mustafa K Khokha; Michael A Choma

doi:10.1038/s41598-017-14670-9

Visualization and quantification of injury to the ciliated epithelium using quantitative flow imaging and speckle variance optical coherence tomography

Sci Rep. 2017 Nov 8;7(1):15115. doi: 10.1038/s41598-017-14670-9.

Authors

Ute A Gamm¹, Brendan K Huang², Emily K Mis³, Mustafa K Khokha^{3

4}, Michael A Choma^{5

6

7

8}

Affiliations

¹ Yale University, Department of Radiology & Biomedical Imaging, Yale University, 333 Cedar Street, New Haven, CT, 06520, USA.
² Yale University, Department of Biomedical Engineering, Yale University, 55 Prospect Street, New Haven, CT, 06511, USA.
³ Yale University, Department of Pediatrics, Yale University, 333 Cedar Street, New Haven, CT, 06520, USA.
⁴ Yale University, Department of Genetics, 333 Cedar St., New Haven, CT, 06510, USA.
⁵ Yale University, Department of Radiology & Biomedical Imaging, Yale University, 333 Cedar Street, New Haven, CT, 06520, USA. michael.choma@yale.edu.
⁶ Yale University, Department of Biomedical Engineering, Yale University, 55 Prospect Street, New Haven, CT, 06511, USA. michael.choma@yale.edu.
⁷ Yale University, Department of Pediatrics, Yale University, 333 Cedar Street, New Haven, CT, 06520, USA. michael.choma@yale.edu.
⁸ Yale University, Department of Applied Physics, Yale University, 15 Prospect Street, New Haven, CT, 06520, USA. michael.choma@yale.edu.

Abstract

Mucociliary flow is an important defense mechanism in the lung to remove inhaled pathogens and pollutants. Disruption of ciliary flow can lead to respiratory infections. Multiple factors, from drugs to disease can cause an alteration in ciliary flow. However, less attention has been given to injury of the ciliated epithelium. In this study, we show how optical coherence tomography (OCT) can be used to investigate injury to the ciliated epithelium in a multi-contrast setting. We used particle tracking velocimetry (PTV-OCT) to investigate the cilia-driven flow field and 3D speckle variance imaging to investigate size and extent of injury caused to the skin of Xenopus embryos. Two types of injuries are investigated, focal injury caused by mechanical damage and diffuse injury by a calcium chloride shock. We additionally investigate injury and regeneration of cilia to calcium chloride on ex vivo mouse trachea. This work describes how OCT can be used as a tool to investigate injury and regeneration in ciliated epithelium.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Cilia / physiology*
Epithelium / embryology
Epithelium / injuries
Epithelium / physiopathology*
Mice, Inbred C57BL
Regeneration
Rheology
Skin / embryology
Skin / injuries
Skin / physiopathology*
Tomography, Optical Coherence
Trachea / diagnostic imaging
Trachea / injuries
Trachea / physiopathology*
Xenopus

Abstract

Publication types

MeSH terms

Grants and funding