Assay for β-Galactosidase in Extracts of Mammalian Cells

Cold Spring Harb Protoc. 2017 Oct 3;2017(10):pdb.prot095778. doi: 10.1101/pdb.prot095778.

Abstract

This protocol contains a method for the detection of β-galactosidase expressed from reporter vectors that have been transfected into mammalian cells. The assay is both simple and rapid and can be performed using a visible light spectrophotometer.

MeSH terms

  • Animals
  • Cell Extracts / isolation & purification*
  • Cell Line
  • Mammals
  • Spectrophotometry / methods*
  • beta-Galactosidase / analysis*

Substances

  • Cell Extracts
  • beta-Galactosidase