Genome-Wide RNA Interference: Functional Genomics in the Postgenomics Era

Katerina Politi; Narendra Wajapeyee

doi:10.1101/pdb.top097550

Genome-Wide RNA Interference: Functional Genomics in the Postgenomics Era

Cold Spring Harb Protoc. 2017 Sep 1;2017(9):pdb.top097550. doi: 10.1101/pdb.top097550.

Authors

Katerina Politi, Narendra Wajapeyee

PMID: 28864574
DOI: 10.1101/pdb.top097550

Abstract

This introduction briefly describes the types of RNAi libraries (both shRNA-based and double-stranded siRNA-based) that are available for understanding diverse biological questions and then discusses recent advances in RNAi screening methodologies in mouse, rat, humans, Drosophila, and worms.

MeSH terms

Animals
Caenorhabditis elegans / genetics
Drosophila melanogaster / genetics
Genome*
Genomics / methods*
Mice
Planarians / genetics
RNA Interference*