Effect of corrosion inhibitor benzotriazole on the uptake and translocation of Cd in rice (Oryza sativa L.) under different exposure conditions

Emerging contaminants that can complex with heavy metals might affect the speciation of coexisting metals and result in different ecological risks. As a widely used metal corrosion inhibitor, 1H-benzotriazole (BTR) is frequently detected in the environments, sometimes at very high levels. In this study, rice (Oryza sativa L.) was used to assess the ecological risk of combined exposure to cadmium (Cd) and BTR in plants and discuss the potential effects of exposure sequence on the uptake and translocation of Cd under hydroponic culture. In the combined exposure treatments, Cd concentration in rice significantly decreased when the molar ratio of BTR to Cd exceeded 1, while the oxidative damage of root was alleviated. In the sequential exposure treatments, an exposure to BTR accelerated the release of preabsorbed Cd from seedlings to the environment and increased the transport of Cd from the roots to shoots at high BTR concentrations. This demonstrates that the combined pollution effect of Cd and BTR is present not only in the environment but also in plants. With the decrease in Cd concentration in the roots, the electrolytic leakages from the roots also decreased, indicating that root damage repair was induced by the subsequent BTR exposure. BTR was mainly accumulated in the seedling roots. Preabsorbed BTR significantly increased Cd concentration in the roots of rice seedlings but inhibited Cd translocation from the roots to shoots of the rice seedlings.