Boosting the Peroxidase-Like Activity of Nanostructured Nickel by Inducing Its 3+ Oxidation State in LaNiO₃ Perovskite and Its Application for Biomedical Assays

Catalytic nanomaterials with intrinsic enzyme-like activities, called nanozymes, have recently attracted significant research interest due to their unique advantages relative to natural enzymes and conventional artificial enzymes. Among the nanozymes developed, particular interests have been devoted to nanozymes with peroxidase mimicking activities because of their promising applications in biosensing, bioimaging, biomedicine, etc. Till now, lots of functional nanomaterials have been used to mimic peroxidase. However, few studies have focused on the Ni-based nanomaterials for peroxidase mimics. In this work, we obtained the porous LaNiO₃ nanocubes with high peroxidase-like activity by inducing its 3+ oxidation state in LaNiO₃ perovskite and optimizing the morphology of LaNiO₃ perovskite. The peroxidase mimicking activity of the porous LaNiO₃ nanocubes with Ni³⁺ was about 58~fold and 22~fold higher than that of NiO with Ni²⁺ and Ni nanoparticles with Ni⁰. More, the porous LaNiO₃ nanocubes exhibited about 2-fold higher activity when compared with LaNiO₃ nanoparticles. Based on the superior peroxidase-like activity of porous LaNiO₃ nanocubes, facile colorimetric assays for H₂O₂, glucose, and sarcosine detection were developed. Our present work not only demonstrates a useful strategy for modulating nanozymes' activities but also provides promising bioassays for clinical diagnostics.