IL-23 Gene and Protein Expression in Childhood Asthma

Iran J Immunol. 2017 Mar;14(1):73-80.

Abstract

Background: Asthma is the chronic inflammation of airways characterized by eosinophilic infiltration, mucus overproduction, airway hyper-responsiveness and airway remodeling. These changes are induced mostly by cytokines which are produced by T helper (Th) 2 cells. Recently, the role of interleukin-23 (IL-23) in the pathogenesis of adult allergic asthma has been studied.

Objective: To explore IL-23 serum levels and its expression in persistent asthma compared with healthy children younger than five years old.

Method: Blood samples of 40 children with mild and severe persistent asthma were compared to 34 healthy children regarding IL-23 serum levels and gene expression using enzyme-linked immunosorbentassay (ELISA) and real time quantitative polymerase chain reaction (PCR).

Results: The IL-23 gene expression level was significantly different in the 25 children with mild persistent asthma and the 15 children with severe persistent asthma compared to the control group (p=0.001). There was no significant difference in IL-23 gene expression level between the two groups of patients with mild and severe persistent asthma. A significant difference was seen in IL-23 serum levels between the 25 children with persistent asthma and control group (p=0.002).

Conclusion: For pre-school children with history and physical exam in favor of asthma which cannot be tested by spirometry, IL-23 serum levels may be an auxiliary biomarker for the diagnosis of asthma.

MeSH terms

  • Adult
  • Asthma / diagnosis
  • Asthma / immunology*
  • Biomarkers / blood*
  • Child, Preschool
  • Disease Progression
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Gene Expression Regulation
  • Humans
  • Infant
  • Infant, Newborn
  • Interleukin-23 Subunit p19 / blood*
  • Interleukin-23 Subunit p19 / genetics
  • Male
  • Polymerase Chain Reaction

Substances

  • Biomarkers
  • IL23A protein, human
  • Interleukin-23 Subunit p19