Arabidopsis receptor-like cytoplasmic kinase BIK1: purification, crystallization and X-ray diffraction analysis

Acta Crystallogr F Struct Biol Commun. 2016 Oct 1;72(Pt 10):738-742. doi: 10.1107/S2053230X16013522. Epub 2016 Sep 22.

Abstract

Receptor-like cytoplasmic kinases (RLCKs) in Arabidopsis play a central role in the integration of signaling input from various growth and immune signaling pathways. BOTRYTIS-INDUCED KINASE 1 (BIK1), belonging to the RLCK family, is an important player in defense against bacterial and fungal pathogens and in ethylene and brassinosteroid hormone signaling. In this study, the purification and crystallization of a first member of the class VI family of RLCK proteins, BIK1, are reported. BIK1 was crystallized using the microbatch-under-oil method. X-ray diffraction data were collected to 2.35 Å resolution. The crystals belonged to the monoclinic space group C2, with two monomers per asymmetric unit.

Keywords: Arabidopsis; BIK1; BOTRYTIS-INDUCED KINASE 1; PAMP-triggered immunity; RLCK; receptor-like cytoplasmic kinases; receptor-like kinase.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / chemistry*
  • Arabidopsis / enzymology
  • Arabidopsis / immunology
  • Arabidopsis Proteins / chemistry*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / immunology
  • Cloning, Molecular
  • Crystallization
  • Crystallography, X-Ray
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Gene Expression
  • Plasmids / chemistry
  • Plasmids / metabolism
  • Protein Serine-Threonine Kinases / chemistry*
  • Protein Serine-Threonine Kinases / genetics
  • Protein Serine-Threonine Kinases / immunology
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • X-Ray Diffraction

Substances

  • Arabidopsis Proteins
  • Recombinant Proteins
  • BIK1 protein, Arabidopsis
  • Protein Serine-Threonine Kinases