Draft Genome Sequence of Agrobacterium rhizogenes Strain NCPPB2659

Jose A Valdes Franco; Ray Collier; Yi Wang; Naxin Huo; Yong Gu; Roger Thilmony; James G Thomson

doi:10.1128/genomeA.00746-16

Draft Genome Sequence of Agrobacterium rhizogenes Strain NCPPB2659

Genome Announc. 2016 Jul 28;4(4):e00746-16. doi: 10.1128/genomeA.00746-16.

Authors

Jose A Valdes Franco¹, Ray Collier¹, Yi Wang², Naxin Huo², Yong Gu¹, Roger Thilmony³, James G Thomson³

Affiliations

¹ USDA-ARS Crop Improvement and Genetics, Western Regional Research Center, Albany, California, USA.
² USDA-ARS Crop Improvement and Genetics, Western Regional Research Center, Albany, California, USA Department of Plant Sciences, University of California, Davis, Davis, California, USA.
³ USDA-ARS Crop Improvement and Genetics, Western Regional Research Center, Albany, California, USA roger.thilmony@ars.usda.gov james.thomson@ars.usda.gov.

Abstract

This work reports the draft genome sequence of Agrobacterium rhizogenes strain NCPPB2659 (also known as strain K599). The assembled genome contains 5,277,347 bp, composed of one circular chromosome, the pRi2659 virulence plasmid, and 17 scaffolds pertaining to the linear chromosome. The wild-type strain causes hairy root disease in dicots and has been used to make transgenic hairy root cultures and composite plants (nontransgenic shoots with transgenic roots). Disarmed variants of the strain have been used to produce stable transgenic monocot and dicot plants.