Fast-Flowering Mini-Maize: Seed to Seed in 60 Days

Genetics. 2016 Sep;204(1):35-42. doi: 10.1534/genetics.116.191726. Epub 2016 Jul 20.

Abstract

Two lines of Zea mays were developed as a short-generation model for maize. The Fast-Flowering Mini-Maize (FFMM) lines A and B are robust inbred lines with a significantly shorter generation time, much smaller stature, and better greenhouse adaptation than traditional maize varieties. Five generations a year are typical. FFMM is the result of a modified double-cross hybrid between four fast-flowering lines: Neuffer's Early ACR (full color), Alexander's Early Early Synthetic, Tom Thumb Popcorn, and Gaspe Flint, followed by selection for early flowering and desirable morphology throughout an 11-generation selfing regime. Lines A and B were derived from different progeny of the initial hybrid, and crosses between Mini-Maize A and B exhibit heterosis. The ancestry of each genomic region of Mini-Maize A and B was inferred from the four founder populations using genotyping by sequencing. Other genetic and genomic tools for these lines include karyotypes for both lines A and B, kernel genetic markers y1 (white endosperm) and R1-scm2 (purple endosperm and embryo) introgressed into Mini-Maize A, and ∼24× whole-genome resequencing data for Mini-Maize A.

Keywords: Zea mays; flowering time; heterosis; maize; model system.

MeSH terms

  • Crosses, Genetic
  • Flowers / genetics
  • Flowers / growth & development
  • Genetic Markers
  • Genotype
  • Genotyping Techniques / methods
  • Hybrid Vigor
  • Karyotype
  • Models, Genetic*
  • Seeds / genetics
  • Zea mays / genetics*
  • Zea mays / growth & development*

Substances

  • Genetic Markers