Particle streak velocimetry-optical coherence tomography: a novel method for multidimensional imaging of microscale fluid flows

Kevin C Zhou; Brendan K Huang; Ute A Gamm; Vineet Bhandari; Mustafa K Khokha; Michael A Choma

doi:10.1364/BOE.7.001590

Particle streak velocimetry-optical coherence tomography: a novel method for multidimensional imaging of microscale fluid flows

Biomed Opt Express. 2016 Mar 30;7(4):1590-603. doi: 10.1364/BOE.7.001590. eCollection 2016 Apr 1.

Authors

Kevin C Zhou¹, Brendan K Huang², Ute A Gamm³, Vineet Bhandari⁴, Mustafa K Khokha⁵, Michael A Choma⁶

Affiliations

¹ Department of Biomedical Engineering, Yale University, 55 Prospect St., New Haven, Connecticut 06520, USA; Current affiliation: Department of Biomedical Engineering, Duke University, Fitzpatrick CIEMAS, 101 Science Dr., NC 27708, USA; kevin.zhou@duke.edu.
² Department of Biomedical Engineering, Yale University, 55 Prospect St., New Haven, Connecticut 06520, USA.
³ Department of Radiology & Biomedical Imaging, Yale University, 333 Cedar St., New Haven, Connecticut 06510, USA.
⁴ Department of Pediatrics, Yale University, 333 Cedar St., New Haven, Connecticut 06510, USA.
⁵ Department of Pediatrics, Yale University, 333 Cedar St., New Haven, Connecticut 06510, USA; Department of Genetics, Yale University, 333 Cedar St., New Haven, Connecticut 06510, USA.
⁶ Department of Biomedical Engineering, Yale University, 55 Prospect St., New Haven, Connecticut 06520, USA; Department of Radiology & Biomedical Imaging, Yale University, 333 Cedar St., New Haven, Connecticut 06510, USA; Department of Pediatrics, Yale University, 333 Cedar St., New Haven, Connecticut 06510, USA; Department of Applied Physics, Yale University, P.O. Box 208267, New Haven, Connecticut 06520, USA; michael.choma@yale.edu.

Abstract

We present a new OCT method for flow speed quantification and directional velocimetry: particle streak velocimetry-OCT (PSV-OCT). PSV-OCT generates two-dimensional, 2.5-vector component (vx ,|vy |,vz ) maps of microscale flow velocity fields. Knowledge of 2.5-vector components also enables the estimation of total flow speed. The enabling insight behind PSV-OCT is that tracer particles in sparsely-seeded fluid flow trace out streaks in (x,z,t)-space. The streak orientations in x-t and z-t yield vx and vz , respectively. The in-plane (x-z plane) residence time yields the out-of-plane speed |vy |. Vector component values are generated by fitting streaks to a model of image formation that incorporates equations of motion in 3D space. We demonstrate cross-sectional estimation of (vx ,|vy |,vz ) in two important animal models in ciliary biology: Xenopus embryos (tadpoles) and mouse trachea.

Keywords: (110.4153) Motion estimation and optical flow; (110.4500) Optical coherence tomography; (120.7250) Velocimetry; (170.3880) Medical and biological imaging; (280.2490) Flow diagnostics.

Abstract

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