High-Speed Super-Resolution Imaging of Live Fission Yeast Cells

Methods Mol Biol. 2016:1369:45-57. doi: 10.1007/978-1-4939-3145-3_4.

Abstract

We describe a step-by-step method for high-speed fluorescence photoactivation localization microscopy (FPALM) of live fission yeast cells. The resolution with this method is tenfold better than spinning disk confocal microscopy.

Keywords: FPALM; Fission yeast; Fluorescence microscopy; Live cell s; Super-resolution.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Targeting
  • Microscopy, Fluorescence / methods*
  • Schizosaccharomyces / cytology*
  • Schizosaccharomyces / genetics
  • Transformation, Genetic