To demonstrate effects of aging visually requires a robust technique that can reproducibly detect small differences in efficiency or kinetics between groups. Investigators of aging will greatly appreciate the benefits of Amnis ImageStream technology ( www.amnis.com/), which combines quantitative flow cytometry with simultaneous high-resolution digital imaging. Imagestream is quantitative, reproducible, feasible with limited samples, and it facilitates in-depth examination of cellular mechanisms between cohorts of samples.
Keywords: Aging; Dendritic cell; Flow cytometry; Image analysis; Immune response; Microscopy; Monocyte; Neutrophil; Signal transduction.