Cryo-EM: Spinning the Micelles Away

Satinder K Singh; Fred J Sigworth

doi:10.1016/j.str.2015.08.001

Cryo-EM: Spinning the Micelles Away

Structure. 2015 Sep 1;23(9):1561. doi: 10.1016/j.str.2015.08.001.

Authors

Satinder K Singh¹, Fred J Sigworth²

Affiliations

¹ Department of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA. Electronic address: satinder.k.singh@yale.edu.
² Department of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA. Electronic address: fred.sigworth@yale.edu.

Abstract

Structural characterization of integral membrane proteins (MPs) demands that the samples be pure, monodisperse, and stable. Detergents are required to extract MPs from the lipid bilayer in which they reside and to stabilize them for downstream biophysical analyses. Some of the best MP-stabilizing detergents pose problems for cryo-EM studies, but in this issue of Structure, Hauer et al. (2015) now offer a solution called GraDeR.

Publication types

Comment

MeSH terms

Animals
Cryoelectron Microscopy / methods*
Membrane Proteins / chemistry*
Membrane Proteins / isolation & purification*

Substances

Membrane Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding