Tissue distribution, excretion, and the metabolic pathway of 2,2',4,4',5-penta-chlorinated diphenylsulfide (CDPS-99) in ICR mice

J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Sep 15:1001:90-7. doi: 10.1016/j.jchromb.2015.07.038. Epub 2015 Jul 31.

Abstract

The tissue distribution, excretion, and metabolic pathway of 2,2',4,4',5-penta-chlorinated diphenylsulfide (CDPS-99) in ICR mice were investigated after oral perfusion at 10mg/kg body weight (b.w.). Biological samples were extracted and separated and, for the first time, were determined by a novel, sensitive, and specific GC-MS method under the full scan and selected ion monitoring (SIM) modes. The results showed that the concentrations of CDPS-99 in the liver, kidneys, and serum reached a maximum after a one-day exposure and that the CDPS-99 concentration in the liver was the highest (3.43μg/g). The increase in the concentration of CDPS-99 in muscle, skin, and adipose tissue was slower, and the concentrations of CDPS-99 achieved their highest levels after 3 days of exposure. It was observed that the CDPS-99 concentration in adipose tissue was still very high (0.71μg/g) after 21 days of exposure, which suggested that CDPS-99 was able to accumulate in adipose tissue. In addition, mouse feces accounted for approximately 75% of the total gavage dose, indicating that CDPS-99 was mainly excreted via mouse feces. Metabolism analysis demonstrated that there were three possible metabolic pathways of CDPS-99 in mice: dechlorination reactions with the formation of tetra-CDPS and hydroxylation and oxidation reactions with the formation of OH-CDPS-99 and chlorinated diphenylsulfone. The present study will help to develop a better understanding of mammalian metabolism of CDPS-99.

Keywords: 2,2′,4,4′,5-penta-chlorinated diphenylsulfide (CDPS-99); Distribution; Excretion; Metabolism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chlorobenzenes / pharmacokinetics*
  • Chlorobenzenes / urine
  • Feces / chemistry
  • Gas Chromatography-Mass Spectrometry
  • Hydrogen-Ion Concentration*
  • Male
  • Mice
  • Mice, Inbred ICR
  • Sulfides / pharmacokinetics*
  • Sulfides / urine
  • Tissue Distribution

Substances

  • 2,2',4,4',5-pentachlorodiphenylsulfide
  • Chlorobenzenes
  • Sulfides