Abstract
Memory CD8(+) T cells are critical for host defense upon reexposure to intracellular pathogens. We found that interleukin 10 (IL-10) derived from CD4(+) regulatory T cells (Treg cells) was necessary for the maturation of memory CD8(+) T cells following acute infection with lymphocytic choriomeningitis virus (LCMV). Treg cell-derived IL-10 was most important during the resolution phase, calming inflammation and the activation state of dendritic cells. Adoptive transfer of IL-10-sufficient Treg cells during the resolution phase 'restored' the maturation of memory CD8(+) T cells in IL-10-deficient mice. Our data indicate that Treg cell-derived IL-10 is needed to insulate CD8(+) T cells from inflammatory signals, and reveal that the resolution phase of infection is a critical period that influences the quality and function of developing memory CD8(+) T cells.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adoptive Transfer
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Animals
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CD8-Positive T-Lymphocytes / immunology*
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CD8-Positive T-Lymphocytes / metabolism
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Dendritic Cells / immunology
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Dendritic Cells / metabolism
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Flow Cytometry
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Gene Expression Profiling
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Host-Pathogen Interactions / immunology
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Immunologic Memory / immunology
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Inflammation / genetics
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Inflammation / immunology
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Inflammation / metabolism
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Interleukin-10 / genetics
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Interleukin-10 / immunology*
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Interleukin-10 / metabolism
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Lymphocytic Choriomeningitis / genetics
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Lymphocytic Choriomeningitis / immunology*
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Lymphocytic Choriomeningitis / virology
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Lymphocytic choriomeningitis virus / immunology*
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Lymphocytic choriomeningitis virus / physiology
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Mice, Inbred C57BL
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Mice, Knockout
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Reverse Transcriptase Polymerase Chain Reaction
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T-Lymphocytes, Regulatory / immunology*
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T-Lymphocytes, Regulatory / metabolism
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T-Lymphocytes, Regulatory / transplantation
Substances
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IL10 protein, mouse
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Interleukin-10