Long-term in vitro cultivation of Borrelia miyamotoi

Ticks Tick Borne Dis. 2015 Mar;6(2):181-4. doi: 10.1016/j.ttbdis.2014.12.001. Epub 2014 Dec 22.

Abstract

Borrelia are fastidious bacteria some of which are difficult to grow in vitro. Here, we report a method for successful continuous in vitro cultivation of the emerging pathogen Borrelia miyamotoi. The type and quantity of serum as well as the atmosphere were critical for successful in vitro cultivation. Optimal growth was achieved using 50% pooled human serum and an atmosphere of 6% CO2.

Keywords: Borrelia miyamotoi; In vitro cultivation; Relapsing fever spirochetes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Atmosphere
  • Borrelia / genetics
  • Borrelia / growth & development*
  • Carbon Dioxide / metabolism
  • Culture Media*
  • Humans
  • Ixodes / microbiology*
  • Larva
  • Mice, SCID
  • Rabbits
  • Relapsing Fever / microbiology*
  • Serum
  • Time Factors

Substances

  • Culture Media
  • Carbon Dioxide