Insights into substrate binding to the oxygen-evolving complex of photosystem II from ammonia inhibition studies

Biochemistry. 2015 Jan 20;54(2):622-8. doi: 10.1021/bi5014134. Epub 2015 Jan 6.

Abstract

Water oxidation in Photosystem II occurs at the oxygen-evolving complex (OEC), which cycles through distinct intermediates, S0-S4. The inhibitor ammonia selectively binds to the S2 state at an unresolved site that is not competitive with substrate water. By monitoring the yields of flash-induced oxygen production, we show that ammonia decreases the net efficiency of OEC turnover and slows the decay kinetics of S2 to S1. The temperature dependence of biphasic S2 decay kinetics provides activation energies that do not vary in control and ammonia conditions. We interpret our data in the broader context of previous studies by introducing a kinetic model for both the formation and decay of ammonia-bound S2. The model predicts ammonia binds to S2 rapidly (t1/2 = 1 ms) with a large equilibrium constant. This finding implies that ammonia decreases the reduction potential of S2 by at least 2.7 kcal mol(-1) (>120 mV), which is not consistent with ammonia substitution of a terminal water ligand of Mn(IV). Instead, these data support the proposal that ammonia binds as a bridging ligand between two Mn atoms. Implications for the mechanism of O-O bond formation are discussed.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Ammonia / metabolism*
  • Models, Molecular
  • Oxidation-Reduction
  • Oxygen / metabolism*
  • Photosystem II Protein Complex / metabolism*
  • Protein Binding
  • Spinacia oleracea / metabolism*
  • Temperature
  • Water / metabolism

Substances

  • Photosystem II Protein Complex
  • Water
  • Ammonia
  • Oxygen