Abstract
We demonstrate that the antibiotic amicoumacin A (AMI) is a potent inhibitor of protein synthesis. Resistance mutations in helix 24 of the 16S rRNA mapped the AMI binding site to the small ribosomal subunit. The crystal structure of bacterial ribosome in complex with AMI solved at 2.4 Å resolution revealed that the antibiotic makes contacts with universally conserved nucleotides of 16S rRNA in the E site and the mRNA backbone. Simultaneous interactions of AMI with 16S rRNA and mRNA and the in vivo experimental evidence suggest that it may inhibit the progression of the ribosome along mRNA. Consistent with this proposal, binding of AMI interferes with translocation in vitro. The inhibitory action of AMI can be partly compensated by mutations in the translation elongation factor G.
Copyright © 2014 Elsevier Inc. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / chemistry*
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / genetics
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Base Sequence
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Binding Sites
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Coumarins / chemistry*
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Coumarins / pharmacology
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Crystallography, X-Ray
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Drug Resistance, Bacterial
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Escherichia coli
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Microbial Sensitivity Tests
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Models, Molecular
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Peptide Elongation Factor G / genetics
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Protein Biosynthesis / drug effects*
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Protein Synthesis Inhibitors / chemistry*
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Protein Synthesis Inhibitors / pharmacology
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RNA Stability*
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RNA, Messenger / metabolism
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Ribosome Subunits, Large, Bacterial / chemistry
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Ribosome Subunits, Small, Bacterial / chemistry
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Staphylococcus aureus / genetics
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Thermus thermophilus
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Coumarins
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Peptide Elongation Factor G
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Protein Synthesis Inhibitors
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RNA, Messenger
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amicoumacin A
Associated data
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PDB/4RB5
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PDB/4RB6
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PDB/4RB7
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PDB/4RB8
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PDB/4RB9
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PDB/4RBA
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PDB/4RBB
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PDB/4RBC
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PDB/4RBD
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PDB/4RBE
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PDB/4RBF
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PDB/4RBG