Modified poly(lactic-co-glycolic acid) nanoparticles for enhanced cellular uptake and gene editing in the lung

Adv Healthc Mater. 2015 Feb 18;4(3):361-6. doi: 10.1002/adhm.201400355. Epub 2014 Aug 25.

Abstract

Surface-modified poly(lactic-co-glycolic acid) (PLGA)/poly(β-aminoester)(PBAE)nanoparticles (NPs) have shown great promise in gene delivery. In this work, the pulmonary cellular uptake of these NPs is evaluated and surface-modified PLGA/PBAE NPs are shown to achieve higher cellular association and gene editing than traditional NPs composed of PLGA or PLGA/PBAE blends alone.

Keywords: cell-penetrating peptide; cellular uptake; gene editing; nanoparticles; pulmonary delivery.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • DNA / administration & dosage
  • DNA-Binding Proteins / chemistry
  • Drug Carriers
  • Epithelial Cells / drug effects
  • Epithelial Cells / metabolism
  • Female
  • Gene Transfer Techniques*
  • Green Fluorescent Proteins / genetics
  • Lactic Acid / administration & dosage
  • Lactic Acid / pharmacokinetics*
  • Lung / cytology
  • Lung / drug effects*
  • Macrophages, Alveolar / drug effects
  • Mice, Inbred BALB C
  • Mice, Transgenic
  • Molecular Sequence Data
  • Nanoparticles / administration & dosage*
  • Nanoparticles / chemistry*
  • Polyglycolic Acid / administration & dosage
  • Polyglycolic Acid / pharmacokinetics*
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Surface Properties

Substances

  • DNA-Binding Proteins
  • Drug Carriers
  • MPG peptide
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Lactic Acid
  • DNA