The Golgi ribbon structure facilitates anterograde transport of large cargoes

Mol Biol Cell. 2014 Oct 1;25(19):3028-36. doi: 10.1091/mbc.E14-04-0931. Epub 2014 Aug 7.

Abstract

In mammalian cells, individual Golgi stacks fuse laterally to form the characteristic perinuclear ribbon structure. Yet the purpose of this remarkable structure has been an enigma. We report that breaking down the ribbon of mammalian cells strongly inhibits intra-Golgi transport of large cargoes without altering the rate of transport of smaller cargoes. In addition, insect cells that naturally harbor dispersed Golgi stacks have limited capacity to transport artificial oversized cargoes. These results imply that the ribbon structure is an essential requirement for transport of large cargoes in mammalian cells, and we suggest that this is because it enables the dilated rims of cisternae (containing the aggregates) to move across the stack as they transfer among adjacent stacks within the ribbon structure.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport
  • Cell Line, Tumor
  • Collagen Type I / metabolism*
  • Drosophila
  • Golgi Apparatus / metabolism*
  • Golgi Matrix Proteins
  • HeLa Cells
  • Humans
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Nocodazole / pharmacology
  • RNA Interference
  • RNA, Small Interfering
  • Tubulin Modulators / pharmacology

Substances

  • Collagen Type I
  • GORASP1 protein, human
  • GORASP2 protein, human
  • Golgi Matrix Proteins
  • Membrane Proteins
  • RNA, Small Interfering
  • Tubulin Modulators
  • Nocodazole