Study on the interaction between albendazole and eosin Y by fluorescence, resonance Rayleigh scattering and frequency doubling scattering spectra and their analytical applications

Spectrochim Acta A Mol Biomol Spectrosc. 2014 May 21:126:135-41. doi: 10.1016/j.saa.2014.01.126. Epub 2014 Feb 7.

Abstract

In pH 3.25-3.35 Britton-Robinson (BR) buffer solution, albendazole (ABZ) could react with eosin Y (EY) to form a 1:1 ion-association complex, which not only results in the quenching of fluorescence, but also resulted in the great enhancement of resonance Rayleigh scattering (RRS) and frequency doubling scattering (FDS). Furthermore, a new RRS spectrum will appear, and the maximum RRS wavelength was located at about 356nm. The detection limit for ABZ were 21.51ng mL(-)(1) for the fluorophotometry, 6.93ng mL(-)(1) for the RRS method and 12.89ng mL(-)(1) for the FDS method. Among them, the RRS method had the highest sensitivity. The experimental conditions were optimized and effects of coexisting substances were evaluated. Meanwhile, the influences of coexisting substances were tested. The methods have been successfully applied to the determination of ABZ in capsules and human urine samples. The composition and structure of the ion-association complex and the reaction mechanism were discussed.

Keywords: Albendazole; Eosin Y; Fluorescence quenching; Frequency doubling scattering; Resonance Rayleigh scattering.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Albendazole / analysis*
  • Albendazole / urine*
  • Antiparasitic Agents / analysis*
  • Antiparasitic Agents / urine*
  • Eosine Yellowish-(YS) / chemistry*
  • Fluorescence
  • Humans
  • Light
  • Limit of Detection
  • Models, Molecular
  • Scattering, Radiation
  • Spectrometry, Fluorescence / methods

Substances

  • Antiparasitic Agents
  • Albendazole
  • Eosine Yellowish-(YS)