Abstract
We have investigated the effect of different maturation stimuli on the ability of mature dendritic cells (DCs) to cross-present newly acquired particulate antigens. Cross-presentation was impaired in DCs matured by treatment with TNF-α, CpG and LPS, but was less affected upon CD40L-induced maturation. The difference could not be explained by decreased antigen uptake or translocation into the cytosol, but decreased cross-presentation ability did correlate with increased phagosomal/lysosomal acidification. Nevertheless, intra-phagosomal degradation of OVA was not increased in matured samples, suggesting that decreasing phagosomal pH may also regulate cross-presentation by a mechanism other than enhancing degradation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigen Presentation / immunology*
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Bone Marrow Cells / immunology
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CD40 Ligand / pharmacology
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Cell Differentiation
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CpG Islands
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Cross-Priming / immunology*
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Dendritic Cells / drug effects
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Dendritic Cells / immunology
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Dendritic Cells / metabolism*
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HeLa Cells
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Humans
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Hydrogen-Ion Concentration
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Lipopolysaccharides / pharmacology
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Lysosomes / drug effects
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Lysosomes / immunology
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Lysosomes / metabolism*
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Mice
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Mice, Inbred C57BL
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Oligodeoxyribonucleotides / pharmacology
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Ovalbumin / metabolism
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Phagocytosis / drug effects
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Phagosomes / drug effects
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Phagosomes / immunology
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Phagosomes / metabolism*
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Primary Cell Culture
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Protein Transport / drug effects
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Tumor Necrosis Factor-alpha / pharmacology
Substances
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Lipopolysaccharides
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Oligodeoxyribonucleotides
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Tumor Necrosis Factor-alpha
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CD40 Ligand
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Ovalbumin