Abstract
Engagement of innate viral sensors elicits a robust antiviral program via the induction of type I interferons (IFNs). Innate defense mechanisms against ssDNA viruses are not well defined. Here, we examine type I IFN induction and effectiveness in controlling a ssDNA virus. Using mouse embryonic fibroblasts (MEFs), we found that a murine parvovirus, minute virus of mice (MVMp), induced a delayed but significant IFN response. MEFs deficient in mitochondrial antiviral signaling protein (MAVS) mounted a wild-type IFN response to MVMp infection, indicating that RIG-I-dependent RNA intermediate recognition is not required for innate sensing of this virus. However, MVMp-induced IFNs, as well recombinant type I IFNs, were unable to inhibit viral replication. Finally, MVMp infected cells became unresponsive to Poly (I:C) stimulation. Together, these data suggest that the MVMp efficiently evades antiviral immune mechanisms imposed by type I IFNs, which may in part explain their efficient transmission between mice.
Copyright © 2013 Elsevier Inc. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / deficiency
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Adaptor Proteins, Signal Transducing / genetics
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Adaptor Proteins, Signal Transducing / metabolism*
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Animals
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Antiviral Agents / immunology*
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Antiviral Agents / metabolism
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Female
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Fibroblasts / immunology
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Fibroblasts / virology*
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Immunity, Innate
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Interferon Type I / immunology*
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Interferon Type I / metabolism
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Mice
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Mice, Inbred C57BL
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Minute Virus of Mice / drug effects
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Minute Virus of Mice / immunology*
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Minute Virus of Mice / pathogenicity*
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism
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Parvoviridae Infections / immunology*
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Parvoviridae Infections / virology
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RNA Polymerase III
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Receptors, Cell Surface
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Virus Replication / immunology
Substances
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Adaptor Proteins, Signal Transducing
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Antiviral Agents
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IPS-1 protein, mouse
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Interferon Type I
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Membrane Proteins
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Nerve Tissue Proteins
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Receptors, Cell Surface
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Robo3 protein, mouse
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RNA Polymerase III