Parvovirus evades interferon-dependent viral control in primary mouse embryonic fibroblasts

Virology. 2013 Jul 20;442(1):20-7. doi: 10.1016/j.virol.2013.03.020. Epub 2013 May 12.

Abstract

Engagement of innate viral sensors elicits a robust antiviral program via the induction of type I interferons (IFNs). Innate defense mechanisms against ssDNA viruses are not well defined. Here, we examine type I IFN induction and effectiveness in controlling a ssDNA virus. Using mouse embryonic fibroblasts (MEFs), we found that a murine parvovirus, minute virus of mice (MVMp), induced a delayed but significant IFN response. MEFs deficient in mitochondrial antiviral signaling protein (MAVS) mounted a wild-type IFN response to MVMp infection, indicating that RIG-I-dependent RNA intermediate recognition is not required for innate sensing of this virus. However, MVMp-induced IFNs, as well recombinant type I IFNs, were unable to inhibit viral replication. Finally, MVMp infected cells became unresponsive to Poly (I:C) stimulation. Together, these data suggest that the MVMp efficiently evades antiviral immune mechanisms imposed by type I IFNs, which may in part explain their efficient transmission between mice.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / deficiency
  • Adaptor Proteins, Signal Transducing / genetics
  • Adaptor Proteins, Signal Transducing / metabolism*
  • Animals
  • Antiviral Agents / immunology*
  • Antiviral Agents / metabolism
  • Female
  • Fibroblasts / immunology
  • Fibroblasts / virology*
  • Immunity, Innate
  • Interferon Type I / immunology*
  • Interferon Type I / metabolism
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Minute Virus of Mice / drug effects
  • Minute Virus of Mice / immunology*
  • Minute Virus of Mice / pathogenicity*
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism
  • Parvoviridae Infections / immunology*
  • Parvoviridae Infections / virology
  • RNA Polymerase III
  • Receptors, Cell Surface
  • Virus Replication / immunology

Substances

  • Adaptor Proteins, Signal Transducing
  • Antiviral Agents
  • IPS-1 protein, mouse
  • Interferon Type I
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Receptors, Cell Surface
  • Robo3 protein, mouse
  • RNA Polymerase III