Genome-wide RNAi screening to identify regulators of oncogene-induced cellular senescence

Methods Mol Biol. 2013:965:373-82. doi: 10.1007/978-1-62703-239-1_25.

Abstract

RNA interference (RNAi) is a powerful research tool that can be used to turn off-or silence-the expression of a specific gene. In recent years, RNAi screening on a genome-wide scale has provided the opportunity to identify factors and pathways involved in complex biological processes in a systematic and unbiased manner. Here we describe a genome-wide RNAi screening strategy to identify genes that are required for a particular type of cellular senescence called oncogene-induced senescence. The approach we describe is a general screening strategy that can be applied to the study of other forms of cellular senescence, including replicative senescence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cell Separation
  • Cellular Senescence / genetics*
  • Genomics / methods*
  • Humans
  • Mice
  • Oncogenes / genetics*
  • RNA Interference*
  • RNA, Small Interfering / genetics
  • Retroviridae / genetics
  • Sequence Analysis, DNA

Substances

  • RNA, Small Interfering