Abstract
The slack (slo2.2) gene codes for a potassium-channel α-subunit of the 6TM voltage-gated channel family. Expression of slack results in Na(+)-activated potassium channel activity in various cell types. We describe the purification and reconstitution of Slack protein and show that the Slack α-subunit alone is sufficient for potassium channel activity activated by sodium ions as assayed in planar bilayer membranes and in membrane vesicles.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Cell Line
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Gene Expression
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HEK293 Cells
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Humans
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Lithium / metabolism
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Potassium Channels / chemistry
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Potassium Channels / genetics*
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Potassium Channels / isolation & purification
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Potassium Channels / metabolism*
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Protein Stability
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Protein Subunits / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / isolation & purification
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Recombinant Fusion Proteins / metabolism
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Sodium / metabolism
Substances
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Potassium Channels
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Protein Subunits
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Recombinant Fusion Proteins
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Lithium
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Sodium