Expression, purification and functional reconstitution of slack sodium-activated potassium channels

J Membr Biol. 2012 Nov;245(11):667-74. doi: 10.1007/s00232-012-9425-7. Epub 2012 Jun 23.

Abstract

The slack (slo2.2) gene codes for a potassium-channel α-subunit of the 6TM voltage-gated channel family. Expression of slack results in Na(+)-activated potassium channel activity in various cell types. We describe the purification and reconstitution of Slack protein and show that the Slack α-subunit alone is sufficient for potassium channel activity activated by sodium ions as assayed in planar bilayer membranes and in membrane vesicles.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cell Line
  • Gene Expression
  • HEK293 Cells
  • Humans
  • Lithium / metabolism
  • Potassium Channels / chemistry
  • Potassium Channels / genetics*
  • Potassium Channels / isolation & purification
  • Potassium Channels / metabolism*
  • Protein Stability
  • Protein Subunits / metabolism
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / isolation & purification
  • Recombinant Fusion Proteins / metabolism
  • Sodium / metabolism

Substances

  • Potassium Channels
  • Protein Subunits
  • Recombinant Fusion Proteins
  • Lithium
  • Sodium