NMR insights into protein allostery

Arch Biochem Biophys. 2012 Mar 15;519(2):223-31. doi: 10.1016/j.abb.2011.10.023. Epub 2011 Dec 16.

Abstract

Allosterism is one of nature's principal methods for regulating protein function. Allosterism utilizes ligand binding at one site to regulate the function of the protein by modulating the structure and dynamics of a distant binding site. In this review, we first survey solution NMR techniques and how they may be applied to the study of allostery. Subsequently, we describe several examples of application of NMR to protein allostery and highlight the unique insight provided by this experimental technique.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Allosteric Regulation
  • Kinetics
  • Movement
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Proteins / chemistry*
  • Proteins / metabolism*

Substances

  • Proteins