Circadian expression of clock genes in mouse macrophages, dendritic cells, and B cells

Brain Behav Immun. 2012 Mar;26(3):407-13. doi: 10.1016/j.bbi.2011.10.001. Epub 2011 Oct 13.

Abstract

In mammals, circadian and daily rhythms influence nearly all aspects of physiology, ranging from behavior to gene expression. Functional molecular clocks have been described in the murine spleen and splenic NK cells. The aim of our study was to investigate the existence of molecular clock mechanisms in other immune cells. Therefore, we measured the circadian changes in gene expression of clock genes (Per1, Per2, Bmal1, and Clock) and clock-controlled transcription factors (Rev-erbα and Dbp) in splenic enriched macrophages, dendritic cells, and B cells in both mice entrained to a light-dark cycle and under constant environmental conditions. Our study reveals the existence of functional molecular clock mechanisms in splenic macrophages, dendritic cells, and B cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • ARNTL Transcription Factors / genetics
  • ARNTL Transcription Factors / metabolism
  • Animals
  • B-Lymphocytes / metabolism*
  • CLOCK Proteins / genetics
  • CLOCK Proteins / metabolism
  • Circadian Rhythm / genetics*
  • Circadian Rhythm Signaling Peptides and Proteins / genetics*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Dendritic Cells / metabolism*
  • Gene Expression
  • Macrophages / metabolism*
  • Mice
  • Nuclear Receptor Subfamily 1, Group D, Member 1 / genetics
  • Nuclear Receptor Subfamily 1, Group D, Member 1 / metabolism
  • Period Circadian Proteins / genetics
  • Period Circadian Proteins / metabolism
  • Photoperiod
  • Spleen / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • ARNTL Transcription Factors
  • Bmal1 protein, mouse
  • Circadian Rhythm Signaling Peptides and Proteins
  • DNA-Binding Proteins
  • Dbp protein, mouse
  • Nr1d1 protein, mouse
  • Nuclear Receptor Subfamily 1, Group D, Member 1
  • Per1 protein, mouse
  • Per2 protein, mouse
  • Period Circadian Proteins
  • Transcription Factors
  • CLOCK Proteins
  • Clock protein, mouse