Lung cancer signatures in plasma based on proteome profiling of mouse tumor models

Cancer Cell. 2011 Sep 13;20(3):289-99. doi: 10.1016/j.ccr.2011.08.007.

Abstract

We investigated the potential of in-depth quantitative proteomics to reveal plasma protein signatures that reflect lung tumor biology. We compared plasma protein profiles of four mouse models of lung cancer with profiles of models of pancreatic, ovarian, colon, prostate, and breast cancer and two models of inflammation. A protein signature for Titf1/Nkx2-1, a known lineage-survival oncogene in lung cancer, was found in plasmas of mouse models of lung adenocarcinoma. An EGFR signature was found in plasma of an EGFR mutant model, and a distinct plasma signature related to neuroendocrine development was uncovered in the small-cell lung cancer model. We demonstrate relevance to human lung cancer of the protein signatures identified on the basis of mouse models.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adenocarcinoma of Lung
  • Adenocarcinoma* / blood
  • Adenocarcinoma* / drug therapy
  • Adenocarcinoma* / genetics
  • Adult
  • Aged
  • Aged, 80 and over
  • Animals
  • Antineoplastic Agents / therapeutic use
  • Biomarkers, Tumor / blood*
  • Blood Proteins / genetics
  • Cell Line, Tumor
  • Enzyme-Linked Immunosorbent Assay
  • ErbB Receptors / blood*
  • ErbB Receptors / genetics
  • Erlotinib Hydrochloride
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Lung Neoplasms* / blood
  • Lung Neoplasms* / drug therapy
  • Lung Neoplasms* / genetics
  • Male
  • Mass Spectrometry
  • Mice
  • Middle Aged
  • Molecular Sequence Data
  • Neuroendocrine Cells / cytology
  • Nuclear Proteins / blood*
  • Nuclear Proteins / genetics
  • Proteins / genetics
  • Proteome*
  • Proteomics
  • Quinazolines / administration & dosage
  • Quinazolines / therapeutic use
  • RNA Interference
  • RNA, Small Interfering
  • Small Cell Lung Carcinoma / blood*
  • Small Cell Lung Carcinoma / drug therapy
  • Small Cell Lung Carcinoma / genetics
  • Thyroid Nuclear Factor 1
  • Transcription Factors / blood*
  • Transcription Factors / genetics

Substances

  • Antineoplastic Agents
  • Biomarkers, Tumor
  • Blood Proteins
  • NKX2-1 protein, human
  • Nkx2-1 protein, mouse
  • Nuclear Proteins
  • Proteins
  • Proteome
  • Quinazolines
  • RNA, Small Interfering
  • Thyroid Nuclear Factor 1
  • Transcription Factors
  • Erlotinib Hydrochloride
  • ErbB Receptors

Associated data

  • GEO/GSE28480