Human natural killer cells expressing the memory-associated marker CD45RO from tuberculous pleurisy respond more strongly and rapidly than CD45RO- natural killer cells following stimulation with interleukin-12

Xiaoying Fu; Yun Liu; Li Li; Qin Li; Dan Qiao; Hui Wang; Suihua Lao; Yanying Fan; Changyou Wu

doi:10.1111/j.1365-2567.2011.03464.x

Human natural killer cells expressing the memory-associated marker CD45RO from tuberculous pleurisy respond more strongly and rapidly than CD45RO- natural killer cells following stimulation with interleukin-12

Immunology. 2011 Sep;134(1):41-9. doi: 10.1111/j.1365-2567.2011.03464.x. Epub 2011 Jun 29.

Authors

Xiaoying Fu¹, Yun Liu, Li Li, Qin Li, Dan Qiao, Hui Wang, Suihua Lao, Yanying Fan, Changyou Wu

Affiliation

¹ Institute of Immunology, Zhongshan School of Medicine, Key Laboratory of Tropical Disease Control Research of Ministry of Education, Sun Yat-sen University, Guangzhou Chest Hospital of Guangzhou, Guangzhou, China.

Abstract

Natural killer (NK) cells are known as innate immune lymphocytes that respond rapidly when challenged by pathogens but little is known about adaptive immune features including memory related to NK cells from human beings. In the present study, we demonstrate for the first time that human NK cells expressing the memory-associated marker CD45RO were persistent in pleural fluid cells (PFCs) from tuberculous patients. CD45RO(+) NK cells produced significantly more interferon-γ and were more cytotoxic compared with CD45RO(-) NK cells from PFCs when stimulated with interleukin-12 (IL-12). Consistently, IL-12 enhanced the expression of granzyme B, CD69, CD25, NKG2D, IL-12 receptors β1 and β2 on CD45RO(+) NK cells from PFCs. Our experiments contribute to a better understanding of the NK cells from PFCs and indicate that human CD45RO(+) NK cells from PFCs expressing a 'memory-like' phenotype may have an important role in defending against infection by Mycobacterium tuberculosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Adult
Aged
Antigens, CD / metabolism
Antigens, Differentiation, T-Lymphocyte / metabolism
CD56 Antigen / metabolism
Cell Count
Cytotoxicity, Immunologic / drug effects
Cytotoxicity, Immunologic / immunology
Female
GPI-Linked Proteins / metabolism
Granzymes / metabolism
Humans
Interferon-gamma / metabolism
Interleukin-12 / pharmacology*
Interleukin-2 Receptor alpha Subunit / metabolism
K562 Cells / immunology
Killer Cells, Natural / drug effects
Killer Cells, Natural / immunology*
Killer Cells, Natural / metabolism*
Killer Cells, Natural / pathology
Lectins, C-Type / metabolism
Leukocyte Common Antigens / metabolism*
Leukocytes, Mononuclear / pathology
Lymphocyte Activation / drug effects
Lymphocyte Activation / immunology*
Lymphocyte Subsets / drug effects
Lymphocyte Subsets / immunology
Lymphocyte Subsets / metabolism
Lymphocyte Subsets / pathology
Male
Middle Aged
NK Cell Lectin-Like Receptor Subfamily C / metabolism
NK Cell Lectin-Like Receptor Subfamily K / metabolism
Pleural Effusion / pathology
Receptors, IgG / metabolism
Receptors, Interleukin-12 / metabolism
Tuberculosis, Pleural / immunology*
Tuberculosis, Pleural / pathology
Young Adult

Substances

Antigens, CD
Antigens, Differentiation, T-Lymphocyte
CD56 Antigen
CD69 antigen
FCGR3B protein, human
GPI-Linked Proteins
IL12RB1 protein, human
IL2RA protein, human
Interleukin-2 Receptor alpha Subunit
KLRC1 protein, human
KLRK1 protein, human
Lectins, C-Type
NCAM1 protein, human
NK Cell Lectin-Like Receptor Subfamily C
NK Cell Lectin-Like Receptor Subfamily K
Receptors, IgG
Receptors, Interleukin-12
Interleukin-12
Interferon-gamma
Leukocyte Common Antigens
Granzymes