Development of new peptide-based tools for studying synaptic ribbon function

J Neurophysiol. 2011 Aug;106(2):1028-37. doi: 10.1152/jn.00255.2011. Epub 2011 Jun 8.

Abstract

Synaptic ribbons are proteinaceous specialized electron-dense presynaptic structures found in nonspiking sensory cells of the vertebrate nervous system. Understanding the function of these structures is an active area of research (reviewed in Matthews G, Fuchs P. Nat Rev Neurosci 11: 812-822, 2010). Previous work had shown that ribbons could be effectively labeled and visualized using peptides that bind to the synaptic ribbon protein RIBEYE via a PXDLS motif (Zenisek D, Horst NK, Merrifield C, Sterling P, Matthews G. J Neurosci 24: 9752-9759, 2004). Here, we expand on the previous work to develop new tools and strategies for 1) better visualizing synaptic ribbons, and 2) monitoring and manipulating calcium on the synaptic ribbon. Specifically, we developed a new higher-affinity peptide-based label for visualizing ribbons in live cells and two strategies for localizing calcium indicators to the synaptic ribbon.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Goldfish
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Organ Culture Techniques
  • Peptides / genetics
  • Peptides / physiology*
  • Photoreceptor Cells, Vertebrate / chemistry
  • Photoreceptor Cells, Vertebrate / physiology
  • Presynaptic Terminals / chemistry
  • Presynaptic Terminals / physiology
  • Protein Binding / physiology
  • Retina / chemistry*
  • Retina / physiology
  • Synapses / chemistry*
  • Synapses / physiology*
  • Zebrafish

Substances

  • Peptides