Related B cell clones that populate the CSF and CNS of patients with multiple sclerosis produce CSF immunoglobulin

J Neuroimmunol. 2011 Apr;233(1-2):245-8. doi: 10.1016/j.jneuroim.2011.01.010. Epub 2011 Feb 24.

Abstract

We investigated the overlap shared between the immunoglobulin (Ig) proteome of the cerebrospinal fluid (CSF) and the B cell Ig-transcriptome of CSF and the central nervous system (CNS) tissue of three patients with multiple sclerosis. We determined the IgG-proteomes of CSF by mass spectrometry, and compared them to the IgG-transcriptomes from CSF and brain lesions, which were analyzed by cDNA cloning. Characteristic peptides that were identified in the CSF-proteome could also be detected in the transcriptomes of both, brain lesions and CSF, providing evidence for a strong overlap of the IgG repertoires in brain lesions and in the CSF.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • B-Lymphocyte Subsets / immunology*
  • B-Lymphocyte Subsets / metabolism*
  • B-Lymphocyte Subsets / pathology
  • Clone Cells / immunology
  • Clone Cells / metabolism
  • Clone Cells / pathology
  • Humans
  • Immunoglobulins / biosynthesis*
  • Immunoglobulins / cerebrospinal fluid*
  • Multiple Sclerosis / cerebrospinal fluid*
  • Multiple Sclerosis / genetics
  • Multiple Sclerosis / immunology*

Substances

  • Immunoglobulins