Mast cell degranulation induced by chlorogenic acid

Acta Pharmacol Sin. 2010 Jul;31(7):849-54. doi: 10.1038/aps.2010.63. Epub 2010 Jun 28.

Abstract

Aim: To investigate the mechanism of chlorogenic acid (CA)-induced anaphylactoid reactions.

Methods: Degranulation of peritoneal mast cells was assayed by using alcian blue staining in guinea pigs, and the degranulation index (DI) was calculated. CA-induced degranulation of RBL-2H3 cells was also observed and assayed using light microscopy, transmission electron microscopy, flow cytometry, and beta-hexosaminidase release.

Results: CA 0.2, 1.0, and 5.0 mmol/L was able to promote degranulation of peritoneal mast cells in guinea pigs in vitro, but it did not increase the degranulation of peritoneal mast cells in CA-sensitized guinea pigs compared with control (P>0.05). Treatment with CA 0.2, 1.0, and 5.0 mmol/L for 30, 60, and 120 min induced degranulation in RBL-2H3 cells in a dose- and time-dependent manner (P<0.01). Under transmission electron microscope typical characteristics of degranulation, including migration of granular vesicles toward the plasma membrane and integration combined with exocytosis, were observed, after CA or C48/80 treatment. Fluorescent microscopy and flow cytometric analysis showed that CA induced concentration-dependent translocation of phosphatidylserine in RBL-2H3 cells. beta-hexosaminidase release in RBL-2H3 cells was significantly increased after incubation with 1 mmol/L CA for 60 min and 5 mmol/L CA for 30 min (P<0.01).

Conclusion: CA induces degranulation of peritoneal mast cells and RBL-2H3 cells in guinea pigs, which might be one of the mechanisms of the generation of anaphylactoid reactions induced by CA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaphylaxis / chemically induced*
  • Animals
  • Cell Degranulation / drug effects*
  • Cell Line, Tumor
  • Chlorogenic Acid / administration & dosage
  • Chlorogenic Acid / toxicity*
  • Dose-Response Relationship, Drug
  • Flow Cytometry
  • Guinea Pigs
  • Male
  • Mast Cells / metabolism
  • Mast Cells / physiology
  • Microscopy, Electron, Transmission
  • Peritoneum / cytology
  • Peritoneum / metabolism
  • Rats
  • Time Factors
  • beta-N-Acetylhexosaminidases / metabolism

Substances

  • Chlorogenic Acid
  • beta-N-Acetylhexosaminidases