Cloning, sequencing and expression analysis of a gene encoding alcohol oxidase in Paenibacillus sp. AIU 311

Yasutaka Sasaki; Michihiko Kataoka; Nobuyuki Urano; Jun Ogawa; Akira Iwasaki; Junzo Hasegawa; Kimiyasu Isobe; Sakayu Shimizu

doi:10.1016/j.jbiosc.2010.01.019

Cloning, sequencing and expression analysis of a gene encoding alcohol oxidase in Paenibacillus sp. AIU 311

J Biosci Bioeng. 2010 Aug;110(2):147-51. doi: 10.1016/j.jbiosc.2010.01.019. Epub 2010 Feb 23.

Authors

Yasutaka Sasaki¹, Michihiko Kataoka, Nobuyuki Urano, Jun Ogawa, Akira Iwasaki, Junzo Hasegawa, Kimiyasu Isobe, Sakayu Shimizu

Affiliation

¹ The United Graduate School of Agricultural Sciences, Iwate University, 18-8, Ueda 3-chome, Morioka 020-8550, Japan.

PMID: 20547358
DOI: 10.1016/j.jbiosc.2010.01.019

Abstract

We have cloned a gene encoding an alcohol oxidase (AOD) specific to aldehyde alcohols from Paenibacillus sp. AIU 311. The AOD gene contains an open reading frame consisting of 618 nucleotides corresponding to 205 amino acid residues. The deduced amino acid sequence exhibits a high similarity to that of manganese superoxide dismutases (SODs). We expressed the cloned gene as an active product in Escherichia coli BL21 cells. The productivity (total units per culture broth volume) of the recombinant AOD expressed in E. coli BL21 is 26,000-fold higher than that of AOD in Paenibacillus sp. AIU 311. The recombinant AOD also exhibits aldehyde alcohol oxidase activity and SOD activity. The recombinant cells described in this study have utility for the production of glyoxal from glycolaldehyde.

MeSH terms

Alcohol Oxidoreductases / genetics*
Base Sequence
Cloning, Molecular*
Gene Expression Regulation, Bacterial / genetics
Gene Expression Regulation, Enzymologic / genetics
Molecular Sequence Data
Paenibacillus / enzymology*
Paenibacillus / genetics*

Substances

Alcohol Oxidoreductases
alcohol oxidase