Abstract
Nasopharyngeal swabs (n = 601) from 278 adult and 323 pediatric patients were tested within 24 h of receipt by cytospin-enhanced direct immunofluorescence antibody testing (DFA) and real-time reverse transcriptase PCR (RT-PCR) using the CDC assay. Cytospin-enhanced DFA detected 230 (84.6%) of 272 swine influenza A PCR-positive results overall but 25 (92.6%) of 27 positive results in patients less than 5 years old and 208 (96.7%) of 215 positive samples with cycle threshold values of <26.
Publication types
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Comparative Study
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Evaluation Study
MeSH terms
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Adolescent
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Adult
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Animals
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Child
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Child, Preschool
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Clinical Laboratory Techniques / methods*
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Fluorescent Antibody Technique, Direct / methods
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Humans
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Infant
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Infant, Newborn
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Influenza A Virus, H1N1 Subtype / isolation & purification*
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Influenza, Human / diagnosis*
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Influenza, Human / virology*
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Nasopharynx / virology
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Sensitivity and Specificity
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Virology / methods*
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Young Adult