Abstract
MicroRNAs (miRNAs) pair with target sequences in the 3' untranslated region of mRNAs to posttranscriptionally repress gene expression. In this study, we report that TNF-mediated induction of endothelial adhesion molecules can be regulated by miRNAs that are induced by TNF. Specifically, E-selectin and ICAM-1 are targets of TNF-induced miRNAs miR-31 and miR-17-3p, respectively. Specific antagonism of these TNF-induced miRNAs increased neutrophil adhesion to cultured endothelial cells. Conversely, transfections with mimics of these miRNAs decreased neutrophil adhesion to endothelial cells. These data suggest that miRNAs provide negative feedback control of inflammation.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Cells, Cultured
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E-Selectin / biosynthesis*
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E-Selectin / genetics
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Endothelial Cells / immunology
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Endothelial Cells / metabolism
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Feedback, Physiological / physiology*
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Gene Expression
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Gene Expression Regulation / immunology*
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Humans
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Immunohistochemistry
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Inflammation / genetics
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Inflammation / immunology
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Intercellular Adhesion Molecule-1 / biosynthesis*
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Intercellular Adhesion Molecule-1 / genetics
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MicroRNAs / genetics*
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Oligonucleotide Array Sequence Analysis
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Reverse Transcriptase Polymerase Chain Reaction
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Transfection
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Tumor Necrosis Factor-alpha / metabolism*
Substances
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E-Selectin
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MIRN17 microRNA, human
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MIRN31 microRNA, human
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MicroRNAs
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Tumor Necrosis Factor-alpha
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Intercellular Adhesion Molecule-1