Abstract
Small Cajal body (CB)-specific RNPs (scaRNPs) function in posttranscriptional modification of small nuclear (sn)RNAs. An RNA element, the CAB box, facilitates CB localization of H/ACA scaRNPs. Using a related element in Drosophila C/D scaRNAs, we purified a fly WD40 repeat protein that UV crosslinks to RNA in a C/D CAB box-dependent manner and associates with C/D and mixed domain C/D-H/ACA scaRNAs. Its human homolog, WDR79, associates with C/D, H/ACA, and mixed domain scaRNAs, as well as with telomerase RNA. WDR79's binding to human H/ACA and mixed domain scaRNAs is CAB box dependent, and its association with mixed domain RNAs also requires the ACA motif, arguing for additional interactions of WDR79 with H/ACA core proteins. We demonstrate a requirement for WDR79 binding in the CB localization of a scaRNA. This and other recent reports establish WDR79 as a central player in the localization and processing of nuclear RNPs.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Motifs
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Animals
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Base Sequence
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Cell Line
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Chromatography, Affinity
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Coiled Bodies / metabolism*
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Coiled Bodies / ultrastructure
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Drosophila Proteins / analysis
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Drosophila Proteins / chemistry
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Drosophila Proteins / metabolism*
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Drosophila melanogaster / genetics
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Drosophila melanogaster / metabolism*
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HeLa Cells
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Humans
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Molecular Sequence Data
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Nucleic Acid Conformation
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RNA-Binding Proteins / analysis
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RNA-Binding Proteins / chemistry
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RNA-Binding Proteins / metabolism*
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Recombinant Fusion Proteins / analysis
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Regulatory Sequences, Ribonucleic Acid
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Ribonucleoproteins / analysis
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Ribonucleoproteins / metabolism*
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Sequence Alignment
Substances
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Drosophila Proteins
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RNA-Binding Proteins
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Recombinant Fusion Proteins
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Regulatory Sequences, Ribonucleic Acid
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Ribonucleoproteins
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WDR79 protein, Drosophila
Associated data
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GENBANK/BK006790
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GENBANK/BK006791
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GENBANK/BK006792
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GENBANK/BK006793