Abstract
All-trans retinoic acid (ATRA) induces granulocytic maturation of WEHI-3B D+ leukemia cells and LiCl enhances this maturation, while WEHI-3B D- cells are non-responsive to ATRA. Transfection of SCL, expressed in D- but absent in D+ cells, into D+ cells, caused resistance to ATRA, while transfection of GATA-1 into D+ cells produced resistance to the combination of ATRA and LiCl. SCL expression in D+ cells did not induce the expression of c-Kit, a putative target gene for SCL. LiCl, known to inhibit some kinases by displacing Mg2+, did not affect tyrosine kinase activity of the cytoplasmic domain of c-Kit.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Benzamides
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Cell Differentiation / drug effects*
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Cell Line, Tumor
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DNA Primers
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GATA1 Transcription Factor / metabolism*
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Granulocytes / cytology
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Granulocytes / drug effects*
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Imatinib Mesylate
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Lithium Chloride / pharmacology
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Mice
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Oncogene Proteins, Fusion / metabolism*
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Piperazines / pharmacology
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Plasmids
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Protein-Tyrosine Kinases / antagonists & inhibitors
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Pyrimidines / pharmacology
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RNA, Messenger / genetics
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Tretinoin / pharmacology*
Substances
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Benzamides
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DNA Primers
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GATA1 Transcription Factor
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Gata1 protein, mouse
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Oncogene Proteins, Fusion
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Piperazines
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Pyrimidines
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RNA, Messenger
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SIL protein, mouse
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Tretinoin
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Imatinib Mesylate
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Protein-Tyrosine Kinases
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Lithium Chloride