Abstract
Twelve ssDNA aptamers specific for a novel recombinant anti-neuroexcitation peptide (ANEPIII) were identified using the SELEX method from a 79-nucleotide ssDNA pool to purify ANEPIII in a more efficient way. To further understand the binding modes between ssDNA and ANEPIII, fully flexible dinucleotides were docked onto the homology-modeled ANEPIII structure. AutoDocking identified favorable binding sites on ANEPIII for nucleotides, which was valuable for designing more potent ligands.
MeSH terms
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Aptamers, Nucleotide / chemistry*
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Aptamers, Nucleotide / pharmacology*
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Cloning, Molecular
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DNA, Single-Stranded / drug effects*
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Electrophoretic Mobility Shift Assay
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Enzyme-Linked Immunosorbent Assay
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Models, Molecular
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Nucleic Acid Conformation
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Recombinant Proteins / chemistry
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Recombinant Proteins / pharmacology
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Scorpion Venoms / chemistry*
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Scorpion Venoms / pharmacology*
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Serum Albumin, Bovine / chemistry
Substances
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Aptamers, Nucleotide
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DNA, Single-Stranded
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Recombinant Proteins
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Scorpion Venoms
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anti-epilepsy peptide, Buthrus martensii
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Serum Albumin, Bovine