Abstract
External guide sequences (EGSs) targeting virulence genes from Yersinia pestis were designed and tested in vitro and in vivo in Escherichia coli. Linear EGSs and M1 RNA-linked EGSs were designed for the yscN and yscS genes that are involved in type III secretion in Y. pestis. RNase P from E. coli cleaves the messages of yscN and yscS in vitro with the cognate EGSs, and the expression of the EGSs resulted in the reduction of the levels of these messages of the virulence genes when those genes were expressed in E. coli.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Adenosine Triphosphatases / genetics
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Bacterial Proteins / genetics
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Bacteriological Techniques
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Base Sequence
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Carrier Proteins / genetics
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Escherichia coli / metabolism*
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Gene Expression
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Genetic Techniques
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Membrane Proteins / genetics
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Ribonuclease P / metabolism
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Yersinia pestis / genetics*
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Yersinia pestis / pathogenicity*
Substances
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Bacterial Proteins
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Carrier Proteins
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Membrane Proteins
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YscS protein, Yersinia pestis
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Ribonuclease P
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Adenosine Triphosphatases
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YscN protein, Yersinia