Abstract
The expression in vivo of FMS transcripts and antigen by neoplastic epithelial cells was demonstrated immunohistochemically or by in situ hybridization in sixteen of seventeen human breast carcinoma specimens and one case of sclerosing adenosis. Expression of CSF-1 receptor (FMS) transcripts and protein was also observed in vitro in two or three breast carcinoma-derived cell lines and was dramatically increased by dexamethasone, a potent glucocorticoid and inducer of mammary epithelial cell differentiation. Immunohistochemical staining with an anti-CSF-1 antibody identified neoplastic epithelial cell co-expression of fms and CSF-1 antigens in more than one-third of the fms-positive invasive carcinoma specimens. These results suggest that autocrine and paracrine interactions of the lymphohematopoietic cytokine CSF-1 and its receptor may participate in the biology of human mammary neoplasms.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Blotting, Northern
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Breast Neoplasms / metabolism*
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Breast Neoplasms / pathology
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Breast Neoplasms / ultrastructure
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Cell Line
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Colony-Stimulating Factors / genetics
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Colony-Stimulating Factors / metabolism*
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DNA, Neoplasm / genetics
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DNA, Neoplasm / metabolism
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DNA, Neoplasm / ultrastructure
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Dexamethasone / pharmacology
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Gene Amplification
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Gene Expression / physiology
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Humans
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Immunohistochemistry
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Nucleic Acid Hybridization
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Polymerase Chain Reaction
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Receptor, Macrophage Colony-Stimulating Factor / genetics
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Receptor, Macrophage Colony-Stimulating Factor / metabolism*
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Receptors, Colony-Stimulating Factor / metabolism*
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Receptors, Colony-Stimulating Factor / physiology
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Transcription, Genetic / drug effects
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Transcription, Genetic / genetics*
Substances
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Colony-Stimulating Factors
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DNA, Neoplasm
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Receptors, Colony-Stimulating Factor
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Dexamethasone
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Receptor, Macrophage Colony-Stimulating Factor