Chloroplastic protein NRIP1 mediates innate immune receptor recognition of a viral effector

Cell. 2008 Feb 8;132(3):449-62. doi: 10.1016/j.cell.2007.12.031.

Abstract

Plant innate immunity relies on the recognition of pathogen effector molecules by nucleotide-binding-leucine-rich repeat (NB-LRR) immune receptor families. Previously we have shown the N immune receptor, a member of TIR-NB-LRR family, indirectly recognizes the 50 kDa helicase (p50) domain of Tobacco mosaic virus (TMV) through its TIR domain. We have identified an N receptor-interacting protein, NRIP1, that directly interacts with both N's TIR domain and p50. NRIP1 is a functional rhodanese sulfurtransferase and is required for N to provide complete resistance to TMV. Interestingly, NRIP1 that normally localizes to the chloroplasts is recruited to the cytoplasm and nucleus by the p50 effector. As a consequence, NRIP1 interacts with N only in the presence of the p50 effector. Our findings show that a chloroplastic protein is intimately involved in pathogen recognition. We propose that N's activation requires a prerecognition complex containing the p50 effector and NRIP1.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adaptor Proteins, Signal Transducing / analysis
  • Adaptor Proteins, Signal Transducing / immunology*
  • Adaptor Proteins, Signal Transducing / metabolism
  • Antigens, Viral / immunology
  • Cell Nucleus / chemistry
  • Chloroplasts / chemistry
  • Cytoplasm / chemistry
  • Immunity, Innate
  • Nicotiana / immunology*
  • Nicotiana / virology
  • Nuclear Proteins / analysis
  • Nuclear Proteins / immunology*
  • Nuclear Proteins / metabolism
  • Nuclear Receptor Interacting Protein 1
  • Plant Diseases / immunology*
  • Plant Proteins / analysis
  • Plant Proteins / immunology*
  • Plant Proteins / metabolism
  • Protein Structure, Tertiary
  • Receptors, Virus / analysis
  • Receptors, Virus / immunology*
  • Receptors, Virus / metabolism
  • Thiosulfate Sulfurtransferase / metabolism
  • Tobacco Mosaic Virus / immunology*
  • Two-Hybrid System Techniques

Substances

  • Adaptor Proteins, Signal Transducing
  • Antigens, Viral
  • Nuclear Proteins
  • Nuclear Receptor Interacting Protein 1
  • Plant Proteins
  • Receptors, Virus
  • Thiosulfate Sulfurtransferase